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Amplification of ndvB gene and biofilm formation studies in Escherichia coli

Hamzah Basil Mohammed, Fatemeh Gomroki, Sudhakar Malla and R. Senthil Kumar

Biofilms are complex microbial communities that grow at interfaces, often at solid-liquid surfaces. Biofilms are usually found on solid substrates submerged in or exposed to some aqueous solution, although they can form as floating mats on liquid surfaces and also on the surface of leaves, particularly in high humidity climates. The main objective of the present study was isolation, purification, and characterization of E.coli. Genomic DNA was extracted from the identified isolate, and analyzed using biochemical characterization. The aim of the present study was to understand the presence of ndvB gene in E.coli which in turn is responsible for antibiotic resistance through biofilm formation. E.coli was isolated for biofilm forming regions. The selected E.coli colonies were sub cultured and purified on EMB Agar plates and were further maintained on Nutrient Agar slants. For further confirmation the selected E.coli strains were morphologically characterized by Gram’s staining and were found to be gram negative, short rods. They were further biochemically characterized by tests such as Indole test, Methyl Red Test, VP test and Citrate test. All the strains were positive for Indole test and Methyl Red test, Voges Proskaeur test and Citrate utilisation test were negative. Hence from these tests it was further confirmed that the isolated strains belonged to the genus Escherichia coli. The ndvB gene was amplified and the biofilm studies were confirmed by ethanol method and primary adherence test. The biofilm assay and the primary adherence assay strongly suggests of the biofilm forming nature of the E.coli.