欧洲实验生物学杂志 开放获取

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Antimicrobial activity and phytochemical analysis of crude extracts of endophytic fungi isolated from Plumeria acuminata L. and Plumeria obtusifolia L.

Ramesha A. and C. Srinivas

Endophytes are the chemical synthesizers inside plants and have been extensively investigated for their endophytic microbial complement. Different parts of Plumeria acuminata and Plumeria obtusifolia were subjected to the isolation of endophytic fungi and identified morphologically and also screened for antimicrobial activity against Staphylococcus aureus, Bacillus cereus, Pseudomonas aeruginosa, Escherichia coli, Salmonella typhimurium and Candida albicans. The identification of the prospective endophytic fungi Colletotrichum gloeosporioides (PAS-1) and Fusarium oxysporum (POS-3) was confirmed by molecular technique of 18s rDNA analysis and were selected for the production of secondary metabolites which were grown in rice medium and extracted with ethyl acetate and were screened for their antimicrobial activity by agar well diffusion method and Phytochemical analysis. The extract of C. gloeosporioides gave effective inhibition to the tested organisms except C. albicans; whereas extract of F. oxysporum gave effective inhibition against tested organisms except P. aeruginosa. Phytochemical screening of the extracts revealed the presence of alkaloids, flavonoids, steroids, phenol and phenolic compounds.