Faten Z. Mohamed, Mohamed M. Omran, Hossam Darwish, Arafa A. Belal, Ramzy E. Rashed
Background: A metabolomic study for determination of some candidate metabolomes derived from nucleosides; 1-methyladenosine (1-MA), 1-methylguanosine (1-MG) and 8-hydroxy-2’-deoxyguanosine (8-OHdG) that suggested as being valuable biomarkers for diagnosis of breast cancer. Method: In this study, the serum samples were collected from 107 female as malignant group, 46 as benign group and 52 as healthy subjects. The serum metabolome were detected and quantified by gas chromatography-mass spectroscopy (GC-MS). The efficiency of candidate metabolomes in diagnosis breast cancer and their combinations with CA15-3 were analyzed using multivariate statistical analysis. Result: The serum concentrations of CA15-3, 1-MA, 1-MG and 8-OHdG were determined; the cutoff values were 31.5 U/l, 23.5 μg/l, 18.5 μg/l and 18.0 μg/l respectively. There are significant differences in mean concentrations of CA 15-3, 1-MA, 1-MG and 8-OHdG in malignant breast diseases patients compared to benign and control group. There are significant correlations between the candidate metabolomes and CA15-3 through different groups of patients with breast malignant diseases. The diagnostic performance analyses of candidate metabolomes and their combinations with CA15-3 gives significant values of area under the curve (AUC) ranged from 0.74 to 0.93 with good efficiency ranged from 60.5% to 88.8%. Conclusion: In this study, the candidate
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