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Genetic stability versus somaclonal variation in tissue culture regenerated olive plants (Olea europea cv. Kroneiki)

Zahra Noormohammadi, Banafsheh Kangarloo-Haghighi, Masoud Sheidai, Farah Farahani and Somayeh Ghasemzadeh-Baraki

Genetic stability versus somaclonal variation was studied in one-year old tissue culture regenerated olive plants by using SSR and ISSR molecular markers. Fifty olive plants including mother plant were used in this study. NJ tree and NeigborNet analysis grouped the studied plants in three distinct groups, which were supported by K-Means clustering and Evanno test. AMOVA and Gst analyses revealed significant molecular difference among these three genetic groups. STRUCTURE analysis showed genetic variability both within and among these groups. The regenerated plants differed in allelic composition and allele frequency. The somaclonal variants obtained may be used in further hybridization and olive breeding program.