生物化学与分子生物学杂志 开放获取

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Genotyping of S and C Mutated Beta Globin Gene: Development of a Set of Primers for Use with Different PCR Systems

Koui Tossea A Stephane, Ernest Sery Gonedele-Bi, Eric Gbessi Adji, Albert A Gnondjui, Berenger Ako Ako, Coulibaly Baba, KīŝĂnĂn A Toure, Ibrahima Sanogo, Ronan Jambou*

Sickle cell disease is a genetic disorder that affects nearly 5% of world population. In ivory coast, SCD is a real problem of health and screening is not systematic after born. Here, we designed a set of primers to detect abnormal hemoglobin S and C which can be used both in conventional and quantitative PCR (by curves combinations analysed). A total of 60 blood samples including 13 AA, 23 AS, 9 SS, 12 SC and 3 CC hemoglobin type were screened using hemoglobin electrophoresis and PCR. The universal control primer HBU/R4 was successfully amplified for all of 60 samples. In conventional PCR, for typing of allele S sensibility and specificity of primers were respectively 86.36% and 87.50%. For allele C, sensibility and specificity of this pair were respectively 53.33% and 91.11%. In qPCR, specificity and sensitivity of primers were greater than 85% for allele S and C specific primers.