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In vitro anti-diabetic activity of seed extracts of Cassia auriculata and Cassia angustifolia

Shravan Kumar Nanumala, Tulasi P. and Errabelli Sujitha

To investigate the anti-diabetic potential of seed extracts of Cassia auriculata and Cassia angustifolia in-vitro. The current study evaluated the Anti diabetic activity of Cassia auriculata and Cassia angustifolia via in-vitro inhibition of Wheat α-amylase and Yeast α-glucosidase using ethanol seed extracts. Preliminary phytochemical analysis was performed with ethanol seed extracts of Cassia auriculata and Cassia angustifolia. The α-amylase inhibitory potentials of the extracts were investigated by reacting different concentrations of the extracts with α-amylase and starch solution while α-glucosidase inhibition was determined by pre-incubating α-glucosidase with different concentrations of the extracts followed by the addition of P-nitro, phenyl, glucopyranoside. The ethanolic extract of Cassia auriculata and Cassia angustifolia exhibited appreciable alpha amylase inhibitory activity with an IC₅₀ values 149.6±0.21μg/ml and 228.8±1.25 μg/ml respectively when compared with acarbose (IC₅₀ values 102.76±0.65 μg/ml). and glucosidase inhibitory activity with IC₅₀ values 134.9±0.54 μg/ml and 170.53±0.59 μg/ml respectively. The results of phytochemical analysis of both extract revealed the presence of flavoniods, polyphenols, triterpenoids and steroids. The observed inhibitions of α-amylase and α-glucosidase suggest that the seed extracts of Cassia auriculata and Cassia angustifolia may be useful in the management of diabetes mellitus. The results of this work clearly indicate the potential of these extracts to manage hyperglycemia.