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Isolation and identification of aniline degrading bacteria from sediments of Kharg island in Persian Gulf

Farshid Kafilzadeh, Roghaye Avatefi Nejad and Farzan Mahmoodi Nejad

Aniline and its derivatives are one of an important group of bio-environmental aminoaromatic contaminants. Their presence in the environment is a serious threat and danger for human health and other beings. These combinations are very resistant against degrading. The purpose of this study was isolation and identification of aniline degrading bacteria from sediments of Kharg Island and investigation of their growth in presence of this substrate. Sampling was done from different regions of Kharg Island sediments in spring and summer. Sample was added in mineral medium containing 4oo mg/lit of aniline concentration and aeration was done every day. In order to acute identification, usual biochemical tests and PCR method were used. Bacterial number was counted in the medium containing aniline and growth curve of bacteria was drawn on the basis of obtained number. MIC test was done too. The abundance percentage of positive-gram bacteria was more than the negative- gram bacteria. Aniline resistant bacteria to such as Corynebacterium, Vibrio, Staphylococcus, Pseudoalteromonas, Marinobacter, Pseudomonas, Photobacterium, Bacillus, Salmonella were isolated and identified. The average of bacteria’s number was 8.9×108 and 2.06×1010 CFU/ml in spring and summer respectively. Photobacterium damselae showed the lowest rate of growth. Results showed that aniline biological degradation by isolates was considerable and the Pseudoalteromonas arctica was selected in 2000 mg/lit MIC range as the superior bacterium.