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Isolation and identification of heavy metal resistant bacteria from petroleum soil of Loni, Ahmednagar

Neha A. Gurave, Vrishali V. Korde, Snehal S. Dhas and Mahesh Disale

Increasing bioremediation processes for soils contaminated by heavy metals is one of the severe concern. For the same, broad range of screening procedures for the bacteria having resistance to heavy metals such as lead, nickel, cobalt, chromium, copper, manganese and zinc was attempted. The current study deals with isolation and identification of heavy metal resistant bacteria from petroleum soil collected from petrol pump of Loni, Ahmednagar, Maharashtra. A strain were characterized on the basis of its morphological and biochemical screening tests. It was identified as Bacillus thuringiensis by 16s rRNA sequencing. The strains showed diverse metabolic pattern of carbon sources and other growth factors. They also show tolerance to other heavy metals,such as copper, lead and nickel. antibiotic resistance pattern of the isolate was studied. This study supplied potential bacterial materials for bioremediation in the future.