欧洲实验生物学杂志 开放获取

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Isolation, Purification and Estimation of Zeatin from Corynebacterium aurimucosum

Pooja P. Patel, Purvi M. Rakhashiya, Kiran S. Chudasama and Vrinda S. Thaker

Plant pathogenic organisms are known to affect plants by secreting enzymes, toxic compounds and phytohormones (IAA, KIN, GA, Zeatin and ABA). In the present study, bacteria were isolated from infected Prunus salicina. It was identified by biochemical tests and rDNA sequencing. It was studied for production of zeatin, a phytohormone known to influence plant growth and development. Conditions for zeatin production were optimized and after 10 days of incubation zeatin was extracted from the broth. The extract was subjected to isolation of zeatin using TLC. The further purification of zeatin was performed using HPLC and tested by immunoassay.