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Profiling metabolites in different day cultures of a root endophyte, Frankia Brunchorst from Casuarina equisetifolia L. using GC-MS-MS

S. Santhi, R. Sumathi, C. Rajeshkannan, P. Manivachakam and S. Murugesan

The GC MS-MS investigation was carried out to identify the biosignalling compounds in Frankia that make the Frankia to enhance nodulation in Casuarina equisetifolia. Mass spectrum investigation of Frankia culture resulted in identification of biosignalling compounds viz., Isoterpinolene eluted at retention time (RT) 12.468, 2, 4, Phenolbis (1, 1-dimethyl ethyl) at Rt 18.126 and 1-Dotriocantanol at Rt 19.105, reported to have free radical scavenging activity. The triterpenes Viminalol, β-Amyrine, betulin identified in Frankia were found to be involved in the protection of oxygen sensitive nitrogenase from oxidation.