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Remediation of Hydrocarbon Contaminated Soil through Microbial Degradation- FTIR based prediction Mohd.

Mohd. Muzamil Bhat, Shiv Shankar, Shikha , Mohammad Yunus and R.N. Shukla

Samples of polluted soil contaminated with used motor oil spillage were collected to study whether such soils may be remediated, i.e. whether the remediation of hydrocarbon contaminated soil is achievable using microbial strain sampled from the same site where from the contaminated soil has been collected for isolating bacterial strains. Sampled soil has been divided in to two sets and the one set of the two was inoculated with the isolated microbial strain (treated sample) and the other set left as such without any inoculation (untreated). The inoculation of microbial strain was performed after assessing the water holding capacity of the sampled soils. FTIR spectra were obtained of the untreated samples and have been referred in the study as zero day. Spectra of treated samples were obtained on 12th day of incubation and on 25th day of incubation. A comparison of FTIR spectra of treated (12th day and 25th day) and untreated soil revealed the presence of new bands pertaining to aliphatic and polycyclic aromatic hydrocarbons including various alcohols, aldehydes and ketones. The isolated bacterial strain seems to have substantial potential to remediate the hydrocarbon contamination of the soil and indicate signs of bioremediation of soils contaminated with used motor oil.