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Study on clinical aspects of SPF chickens infected with Ornithobacterium rhinotracheale followed by H9N2 avian influenza

Aidin Azizpour, Hossein Goodarzi, SaiedCharkhkar, Reza Momayez and Mohammad HassanHablolvarid

The aim of this study was to characterize the clinical aspects of ORT followed by H9N2 virus in SPF chickens. Forty two one-day-old SPF chicks were divided randomly into two groups (21 chicks in the experimental and 21 chicks in the control group). At the age of three weeks, the chicks in the experimental group were inoculated intratracheally with 1× 1010 LD50 of ORT-R87-7/1387, three days later, 1×106 EID50 of A/chicken/Iran/m.1/2010 (H9N2) was administrated intra-ocularly. Each bird in control group was inoculated with sterile PBS intra-ocularly. Chickens in each groups was evaluated from 2 to 14 days post inoculation. The results of this study indicated that the ORT followed by H9N2 virus cannot cause mortality, and only slight clinical signs and gross lesions such depression, ruffled feathers, tracheal congestion and pneumonia were seen in infected chickens