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The effect of different concentrations of NAA and BAP on micropropagation of Alstroemeria

Seyyed Rahim Seyyedyousefi, Behzad Kaviani1 and Naghi Padasht Dehkaei

Alstroemeria (Alstroemeriaceae) is an important ornamental plant, which use as cut flower as well as pot and garden plants because of their beautiful attractive flowers with wide variations in flower color. Traditionally, it is propagated via rhizome splitting, but multiplication rate is low and time-consuming process. Micropropagation by rhizome meristems culture is an efficient in vitro propagation method for Alstroemeria. Apical and lateral buds on rhizome were used as explants. These explants were cultured in MS medium containing NAA (0, 0.2, 0.5 and 1.0 mg l-1) and BAP (0, 0.5, 1.0, 1.5 and 2.5 mg l-1). MS medium supplemented with 1.0 mg l-1 NAA resulted in the highest shoot length (6.30 cm), root length (4.86 cm), maximum root number (5.00) and bud number (3.00). Largest number of shoot (3.00) and rhizome (4.00) were obtained in MS medium containing 0.20 and 0.50 mg l-1 NAA.