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The effect of resistance training on serum leptin levels in sedentary overweight females.

Sanaz Mirzayan Shangani, Bahram Abedi, Ardeshir Zafari

Leptin is a regulator of energy intake and exercise training is an increaser factor of energy expenditure. Association of these factors can be new view in prevention and control of obesity. The purpose of this quasi-experimental study was too determined and compared of the effect of 12 weeks incremental resistance training on serum leptin levels in sedentary, overweight females. Twenty five subjects randomly selected from the 40 sedentary (Physical Activity Rating ≤ 2), overweight (30 > Body Mass Index (kg.m^-2) ≥25), volunteers (19-25 yrs.) based on a health and disease risk questionnaire and level of physical activity [based on American College of Sports Medicine (ACSM) and Physical Activity Rating (PA-R) Questionnaires]. This subjects randomly divided in two groups such as, Exercise (BMI: 28.02 ± 3.65; %BF: 41.36 ± 3.64; n= 15) and Control groups (BMI: 27.43 ± 1.25; %BF: 40.23 ± 3.53; n= 10). The independent variable was incremental resistance training based on progressive overload training principal. Training program was performed for 12 weeks, 3 days/week and 90 min/ days. Total time of training program divided as warming up (15 min), main training program (70 min) and cooling down (5 min) at the morning of days (10 – 11.30 am). Training program was started at 60% of One Repetition Maximum (1-RM) at the beginning week and 95% of 1-RM at last week. The dependent variables included fasting serum concentration of Leptin, Body Fat percent (%BF), Body Mass Index (BMI), and Waist to Hip Ratio (WHR) measured at beginning and the end of training program in two groups. Fasting blood sample was taken for measuring serum concentration of leptin after 9 to 12 hours of fasting, 7-8 am, from left Antecubital vein at medical diagnosis laboratory. Leptin measured by ELISA from sandwich competitive method type using DRG-Diagnostica, GmbH, Germany kits. Body Fat percent estimated by three-site (Triceps, Suprailiac and/or Suprailium, and Mid-thigh) skin fold method (Jackson et al. Formula); by used a Harpenden caliper. Body mass index (BMI) calculated by used Quetelet Index (W/H²). Waist to Hip Ratio (WHR) was calculated by dividing the waist circumference in centimeters (cm) by hip circumference in centimeters (cm); by used an inelastic Tape Technique. A two-tailed independent samples t test was used for comparing of means between the exercise and control groups. Within groups comparisons of means were done by used a two-tailed paired samples t test. Significant levels in all tests were set at P≤0.05. Incremental resistance training was reduced body fat percent and serum leptin levels in sedentary, overweight females. Therefore, it seems that leptin changes by resistance training are related to body fat reduction.